DNA

Part:BBa_K4663022:Design

Designed by: Lorenzo da Silva Saraiva   Group: iGEM23_WageningenUR   (2023-10-12)


CEN-like 2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 17
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 17
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 527
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 17
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction sites on both ends of the sequence



Source

Designed in Snapgene based on online available sequences, optimised via the codon optimisation tool of IDT and ordered as a gBlock from IDT as well.


References